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KMID : 1145520180040020090
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Journal of Radiopharmaceuticals and Molecular Probes
2018 Volume.4 No. 2 p.90 ~ p.94
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Preparation of radiolabeled polycyclic aromatic hydrocarbon assemblies for biological assessment of diesel exhaust particulates
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Lee Chang-Heon
Shim Ha-Eun
Song Lee
Jeon Jong-Ho
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Abstract
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The potential health risk from inhalational exposure of diesel exhaust particulates (DEP) has gained considerable scientific interests. However, the long-term in vivo behavior of DEP have not been clearly understood due to the difficulty of accurate analysis of these substances in a living subject. We herein demonstrate a detail protocol for the preparation of radiolabeled DEP using a radioactive-iodine-tagged pyrene analog. The purified 125I-labeled pyrene ([125I]1) was obtained with a good radiochemical yield (32 ± 4%, n=3) and high radiochemical purity (>99%) from the stannylated precursor 2. Next, the purified [125I]1 was successfully assembled into the DEP suspension in an efficient manner. The radiolabeled DEP was highly stable in a mouse serum for 7 days without significant deiodination or dissociation of [125I]1. These results clearly indicate that the present radiolabeling method will be useful for biodistribution study of carbonaceous particulates in vivo.
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KEYWORD
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Radiolabeling, Radioactive iodine, PAHs, DEP
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